I did one transfer which was BFN unfortunately. But actually, it would be 3/7 that were normal, according to how the stats are actually calculated - those that didn't make it to blast were abnormal, most likely. Many couples opt for PGS to provide a higher chance of a successful pregnancy and live birth. I have an older son so I wouldn't mind using an ed. One great, three good, and one fair quality. The PGT biopsy is a fairly safe procedure when done on blastocyst embryos. Why didn't I get pregnant with genetically normal embryos? One such screening is PGS (preimplantation genetic screening) and PGD (preimplantation genetic diagnosis). 2 of the 5 blasts were deemed 'chaotic profile'. Use our. In a woman under age 30, about half of embryos are aneuploid. BFN. March 24, 2016: FET# of 1 blastocyst. Guide to IVF Embryo Grading - Natalist Because embryos are so fragile, the process used for PGS matters a great deal. Should you screen your embryo for genetic abnormalities? I don't have anything very useful to say but I want to at least send you a cyber hug. Diagnosis - Advanced age and PCOS. At age 38, I had 5 blastocysts and only 1 was PGS normal. Success rates for frozen embryo transfer after PGT. Hey Peg, In this post well learn more about IVF with PGS success rates for euploid embryos. In women between the ages of 35-38, only about 50% of embryos will be euploid. Use of this site is subject to our terms of use and privacy policy. Please whitelist our site to get all the best deals and offers from our partners. Neal et al. Me- 32 33 34, DH - 33 34.35.Two Failed IUIs (2015). They found a reduction in live birth rates (50% to 39%), although this was notstatistically significant(it was from a small study). What Is PGT-A or PGS Genetic Screening? It does not look for genetic conditions caused by single mutations in a gene. Preimplantation Genetic Screening (PGS): Top 5 Myths - ORM Genomics Hi I'm I am curious to learn if you chose to do pgs testing what your results were I'm currently awaiting my second round of IVF pgs result. Congratulations on your pregnancy! Miscarriages are defined as the natural death of an embryo or foetus. So in the end 3 of 15 were normal - pretty much bang on the stats. Did you use a sperm donor? *TW* I'm now 31 weeks pregnant from our first FET. those that have reached the blastocyst stage - makes an ongoing pregnancy more likely. Following the miscarriages, she also learned that immunological issues and other complications contributed to her infertility. may be contradicted by other studies. Wish I read it long before my first cycle. 10 mature. However, live birth rates are not necessarily higher with PGS. We do have one normal PGS tested frozen embryo from prior cycle. Embryos with normal PGT results (of any type) can still fail to implant or result in a miscarriage. I took this for about 2 wks before my transfer so now I will take consistently moving forward. Diagnosis: Repeated Pregnancy Loss + DOR + Advance maternal Age. Good to know the process itself works, but decide to bank & do PGS testing in 2 more rounds to try to find a good egg and avoid another painful emotional battle of hope and despair when a pregnancy is not viable. I have a whole page dedicated to mosaic embryos. They concluded that (1) 33% to 50% of all embryos screened in women aged 18 to 48 years are aneuploid and (2) the number and percentage of euploid embryos decreases with advancing maternal age. Common conditions it can screen for include cystic fibrosis, spinal muscular atrophy, Tay-Sachs disease, thalassemia, sickle cell anemia, Huntingtons disease, BRCA, and much more. For the last round, I ate a higher protein diet ( esp plant based proteins), organic foods, Omegas, etc. 144 abnormal (aneuploid/mosaic) embryos and their outcomes. So most <35 women are between 30-90% chance ofeuploid(61% is the average). PGS/PGT-A success rates can vary. Embryo Grading and Success Rates Once fertilized, embryos are graded according to their form and quality. I had 30 eggs, 21 mature, 20 fertilized, 8 made it to blast (6 of them on day 5 and 2 on day 6) Tested all 8: 4 boys, 4 girls. I have some immune issues related to my losses I think so I'm betting the doctor would recommend putting a girl embie back in first. PGS results take between 10-14 days after the embryo biopsy is taken. Preliminary studies examine the rebiopsy and transfer of chaotic embryos by PGT-A. We have decided to do one more round of IVF but first I'm going to try improving my egg quality naturally for the next few weeks. Re-analysis of 166 embryos not transferred after PGS with advanced 2011(38) : Get married and our son is born. However, the classic Hamletian dilemma, 'to PGS or not to PGS', is not. He provides weekly summaries of the latest IVF research and posts on Facebook, Instagram and TikTok regularly. A Group Owner is a member that has initiated the creation of a group to connect with other members to share their journey through the same pregnancy & baby stages. C. Hanson, T. Hardarson, K. Lundin, C. Bergh, T. Hillensj, J. Stevic, C. Westin, U. Selleskog, L. Rogberg, M. Wikland, Re-analysis of 166 embryos not transferred after PGS with advanced reproductive maternal age as indication. If you want to read more about about success rates for untested embryos, go to my embryo grading and success rates post. They also reported the number ofblastsbiopsied. Hi, i know this is an old post but thats what happening to me. Those 4 included 2/6 of the day 5 blast and 2/2 day 6 blast. Why do Chromosomally Normal Embryos Not Implant in the Uterus If you do PDG that does NOT test all 23 chromosomes--some tests only look at the ones that are most commonly a problem, such as 21 (Down's) and a few others--the false positive rate is higher because other chromosomes that don't get tested could be bad. I also change up the meds from Gonal F to Rekovelle. BFN. Not exactly! The most common scale uses a number and two letters to describe each embryo ('4AB' for example). Pricing varies by fertility clinic, and determining the exact cost of PGS/PGT-A testing will vary from patient to patient. In vitro fertilization success rates decline with age, but many women in their early and mid-40s can still conceive with their own eggs, if they are given appropriate fertility treatment. For women ages 41 to 43 the live birth rate drops to 45%. They retrieve 29 eggs 22 of those eggs were mature out of the 22 -19 fertilized and 9 made it to day 5 and were able to be Frozen and biopsied. I just expected better results. In a recent study of 98 women who had mosaic embryos, 32 (33%) elected to have at least one transferred. The term "recurrent miscarriage" means at least two consecutive pregnancies ended prematurely; this affects between 1% and 3% of all women. How many embryos test PGS normal? - Question and Answer - noodles.pildid.eu More studies need to be done. Several functions may not work. Please select a reason for escalating this post to the WTE moderators: Connect with our community members by starting a discussion. 79% of the eggs studied from women 40-45 years old were found to have an abnormal spindle appearance and at least one chromosome displaced from proper alignment. High cellularity of trophectoderm biopsy adversely affects pregnancy outcomes.. Women in the age category above . May 1st- blood test come back positive for hyperthyroidism. FET#1 Jan 2017 - BFN. Women aged 40 and over should always opt for PGS/PGD if it is an option at their clinic because the risks are so high. In women under the age of 35, approximately 60% of embryos will be chromosomally normal. ttc 1 year, 1st ivf, 5 made to day 5. If you have the resources it might make sense to try again. 1st FET; BFP--only 5 weeks and praying it sticks. Some are faster, and some are slower. I think that helped it stick. A blastocyst has two distinct cell lines : A PGT biopsy takes a small sample of 5-7 cells from the trophectoderm. You would only do PGT-M if there is a genetic condition that runs in your family, or if you and your partner carry the same genetic disorder that you risk passing on to your offspring. (2018)found a reduction in clinical pregnancy when embryos were thawed, biopsied and re-frozen (the odds were about half). Further testing - the RPL test- results in suspected immune issues. I had 13 blasts tested from 3 cycles. Cleared to start IVF. Determining IVF with PGS success rates is possible, but contextually its important to realize that without it, embryos that have chromosomal abnormalities can be transferred fresh, or frozen for later transfer. So maybe youve had a cycle and your embryos are frozen, and now you want them biopsied. Criticisms of PGS - FertilityIQ In this patient population, PGT-A offers the benefit of choosing the single best embryo for the transfer. (2016)found nostatistically significantdifference inongoing pregnanciesbetween Day 5 and 6 euploids (78.6% vs 67.4%), but this was reduced by Day 7 (43.8%). This is when the biopsy will be performed and sent for genetic testing. Third one in the past few months doing a medicated cycle. PMID: 30246223; PMCID: PMC6338591. This term has been replaced with PGT-A, or preimplantation genetic testing for aneuploidy. How PGS can Improve Success Rates with Chromosomally Normal Embryos Couples, where one or both partners is a carrier of chromosomal translocation, should also consider PGS. For some women going through IVF, the choice of having PGS performed on their embryos is a no-brainer. That is why we always recommend that you get some form of non-invasive prenatal aneuploidy assessment in early pregnancy at the very least. Percent euploidy was subsequently assessed by day of blastocyst biopsy (days 5, 6, or 7) for each SART age category (Fig. Implantation Failure in IVF - Why Does It Happen? I did all the Sourcing and they are all about quality and science based brands, no Chinese brands as much as possible. DS #1 conceived naturally, born Dec. 1st, 2008 Oftentimes, we will recommend that you see a genetic counselor to understand the risks involved with using a mosaic. IVF: Approach to Selecting the Best Embr yos for Transfer to the Uterus. Damaged embryos may not implant, or could ultimately result in irreparable harm leading to miscarriage and IVF failure. Reminder: I have an integrated glossary in the text (terms are underlined with a dotted black line, and when you tap on it a window will pop up with the definition). It's very interesting. For these groups, about 50% of biopsies had noeuploidembryos. Does it still matter? Underlined Is what I will be adding. The day following your retrieval, we will check to see if fertilization successfully took place. We had 12 embryos PGS tested. For more up-to-date information on this topic check out my other posts that are tagged withEmbryo rebiopsy. Increasing maternal age is associated with decreasing egg quality. But that is on a per-transfer basis. Your email address will not be published. Zhang L, Wei D, Zhu Y, Gao Y, Yan J, Chen ZJ. eggs will be fertilized with sperm on that day, make it to the blastocyst stage on Days 5, 6, or 7. Most of us have 46 chromosomes. Having a euploid embryo does not guarantee a live birth. Success with PGT normal embryo - IVF Support Group *Note: PGS stands for preimplantation genetic screening. Dec. 2nd 2 embryos transferred 5-d-t. Also mild OHSS diagnosis. An embryo could even have a monosomy on one chromosome and a trisomy on another one. In a study analyzing 4,515 patients with up to three consecutive Single Embryo Transfers (SET) of chromosomally normal, or euploid, embryos, 94.9% achieved a pregnancy. And for someone that was willing to donate to a friend you really really deserve it! PGS screens for numerous different genetic diseases that involve chromosome number abnormalities. Those clinics may specialize in IVF and PGS/PGD, and it is worth looking into how a clinic performs the screening. The inner cell mass is what will form the embryo. Preimplantation Genetic Testing - FAQ | Fertility & Reproductive Those who do not seek counsel from the appropriate health care authority assume the liability for any damage, loss, or injury which may occur. Complete guide to PGS Testing (PGT-A) - Remembryo With that said, nothing is ever 100%. Doctor hinted I might be cleared to go back to IVF as soon as September. When looking at IVF success rates, its important to consider all of the success metrics, not just miscarriage rates. If they've got a few embryos, they might test them all to help pick the one with the best chance at becoming a pregnancy. In women aged 27 to 35 years, the median proportion of euploid embryos in each cycle remained constant at approximately 35% in day-3 biopsies and approximately 55% in day-5 biopsies, but it decreased rapidly after age 35. Aneuploidy in embryos by maternal age <25 - 36% 25-30 - 40% 31-34 - 43% 35-37 - 51% 38-40 - 64% 41-42 - 76% Chance of at least one normal embryo 25-30 - 91% 31-34 - 89% 35-37 - 83% 38-40 - 68% 41-42 - 51% 15 suran88 1 yr. ago Awesome Thank you so much for sharing this 2 chivy_2338 2 mo. February 2012 IVF #1 -BFN. Normal Embryo Success Rate: RMA Research Study Demko ZP, Simon AL, McCoy RC, Petrov DA, Rabinowitz M. Effects of maternal age on euploidy rates ina large cohort of embryos analyzedwith 24-chromosome single-nucleotide polymorphism-based preimplantation genetic screening. This educational content is not medical or diagnostic advice. Thanks everyone, I feel a little less panicked now, it was just not what I was expecting! 4 came out normal. We added 12 hours of progesterone. That's unfortunate to know. Up to age 40, most women will have at least one normal embryo. Nov. 28th- Embryo update: 12 eggs retrieved. 1 frozen (testing later revealed it was abnormal). Am I being overly paranoid? You should not rely solely on this information. Instead of seeing two copies of chromosome 21, an embryo with Down syndrome will have three copies (Trisomy 21). Epub 2016 Feb 8. Factors that can influence treatment costs include: This can be done! Across all maternal ages, the euploid proportion and number of embryos per cycle were counterbalanced, so the number of euploid embryos per cycle was the same for day-3 and day-5 biopsies. I have had MaterniT Genome done and there were no chromosomal abnormalities found at 10 weeks, and all the NT scans and anatomy scans have resulted in a normal healthy baby boy. 75% of embryos are abnormal by age 42, and 90% by age 44 Some studies have shown that there is a higher percentage of chromosomal abnormalities in day 3 embryos than in day 5 embryos. PGT-M (preimplantation genetic testing for monogenic diseases) takes it one step deeper and screens for specific genetic conditions that affect individual genes. Fresh versus Frozen Embryo Transfers (FET) Enhance IVF Outcome. PGT cannot detect autism because it is not caused by a single gene mutation or a chromosomal numerical abnormality. January 2010, Begin TTC #2 We retrieved 19, 17 fertilized and 10 made it to day 5. So those 2 could have been normal but they didn't get a reading on them. In this case the clinic will need to: So the embryo would have to go through multiple rounds of freezing/thawing/biopsy, and this might have an impact on its potential. Abnormalities lead to failed natural and assisted pregnancies, and that is the primary reason PGS increases IVF success. This is not recommended for shared computers. Create an account or log in to participate. PGT-A: Guide to Preimplantation Genetic Testing of Embryos in IVF No problems found. The overall pregnancy rate per IVF cycle that uses PGS testing is 71%, according to a study of 241 embryos with an average maternal age of 35.7. Abnormal embryos, called aneuploid, would not have this normal complement of chromosomes. This is a fancy way of saying, does the embryo contain the correct number of chromosomes? Does this harm the embryo or reduce its potential for success? We had 6 out of 10 retrieved make it to day 5/6. IVF with PGS Success Rates: Who Benefits from PGS/PGD Congratulations, that's such a heartwarming story. If the embryo is missing a chromosome and has 45, this is known as monosomy. On average, women in their late 20s had four euploid embryos (day 3 or day 5) per cycle, but this number decreased linearly (R2 R 0.983) after 35 years of age. Here is some more interesting research that is brand new out of MIT. percentage of pgs normal embryos by age - VintageRPM I am 40 and had to do many, many rounds of IVF (6) before I got any PGS normal. The live birth rate declines to 55% for women ages 35 to 40. I had no more normal boys left but did have 1 abnormal boy left (mosaic monosomy 6). The team hope that the research will . However, PGT-A does not seem to improve overall pregnancy rates in women under the age of 35. December 2015: IVF Retrieval #3 - 31 retrieved, 24 mature, 21 fertilized w/ICSI, 8 frozen, 3 normal. Several situations pose a certain risk to PGS: PGS/PGD can provide an additional layer of assurance that IVF has higher success ratesin older women who generally have a greater chance of failed treatment. So here is what I will be taking. By 40 theres about a 1 in 4 chance of not getting a euploid and by 43 this doubles to half. So that puts us actually below 50% normal. We expected to have at least one. Liebermann et al. Two IUIs. 2: Blastocystthe blastocele is greater than or equal to half of the volume of the embryo. These forums were a critical source of support when we were in the midst of fertility treatments, and even now as I struggle with accepting that it is over. I'm pretty sure they dispose immediately, as that is what I was told by my office. Thought just because your embryo iseuploidthat grades dont matter anymore? I will be using the terms interchangeably.*. But that is on a per-transfer basis. Huge difference. My Dr says at my age about 75% will be abnormal. Now, I can't source that, but it was something one of the REs that did an AMA on here said - maybe Dr. Aimee? Some clinics have extremely high IVF success rates. Note that this is per transfer data. Currently, using this technology, we are having pregnancy rates approximating 70% with the transfer of a single embryo. At 35 years of age, about 50% of a woman's eggs are chromosomally normal. Because of this, women fertility clinics can reduce the likelihood of miscarriage through PGS/PGD, that is great news. Preimplantation Genetic Screening: How Age Affects Number of Eggs Embryos end up with an abnormal number of chromosomes because the egg has abnormal chromosomes, or the sperm has abnormal chromosomes or during the first cell division after fertilization, the chromosomes are inappropriately separated. Your embryos will then be kept in the IVF lab for one week. Transfers using excellent grade blastocysts have a pregnancy rates of about 65%. For now its probably best to avoid having to thaw and biopsy if possible. Group Leaders communicate with staff moderators and escalate potential violations for review, but they dont moderate discussions. I know how hard it is. Unfortunately, 1 did not survive the thaw, and none of the other 3 resulted in a successful pregnancy. All four arrested embryos diagnosed as normal at PGS analysis were shown to be chromosomally overall abnormal. This is because chromosomally abnormal embryos are more likely to arrest during early development compared to chromosomally normal embryos It is now clear that for women of all ages, PGT-A leads to higher pregnancy rates than we have ever experienced previously. 3 C). Beta November 7th. Consult with your doctor before making any treatment changes. Our second and last IVF we just got one blastocyst as well and are waiting for our PGS results. Our RE told us that once you've had an all-abnormal cycle, doing one more cycle can make sense, but if that second cycle is also all-abnormal, the chances of any normals after that are so incredibly small that in her view it is unethical for a clinic to do a third cycle. We opted to not know the gender. We sent the best 8 for testing. Normal embryos are called euploid and contain 46XX if female or 46XY if male. To see a fertility specialist with a high success rate using single embryo transfer, make an appointment at one of InVia's four Chicago area fertility clinics. END MENTS We have 1 more normal embryo. Newport Beach, California 92663. Success rates with chromosomally normal embryos, complete guide to embryo grading and success rates, They also found that the chance of getting a complex abnormal embryo increased with age, Graded as excellent (AA) or good (AB or BA) = 50% live birth, Graded as excellent: 84%ongoing pregnancy, 0% miscarriage, Good: 62%ongoing pregnancy, 3% miscarriage, Poor: 36%ongoing pregnancy, 19% miscarriage, Thaw the embryo and culture it until it can be biopsied (this may take a few hours to a day), If the 2nd test comes back euploid and you transfer, another thaw will need to be done. We found out yesterday the PGS results were poor quality eggs due to my age. https://alexroblesmd.com/wp-content/cache/breeze-minification/js/breeze_d8b9ac1cac0e674c1a0b0961093927ba.js, https://alexroblesmd.com/wp-content/cache/breeze-minification/js/breeze_e709f6277bbec007e5a021ac9cdc419b.js, https://alexroblesmd.com/wp-content/cache/breeze-minification/js/breeze_d6638419dc0ffa7ebd981022572d700a.js, https://alexroblesmd.com/wp-content/cache/breeze-minification/js/breeze_b410f7096d4a966b622520512b7f5e7d.js. I have one left so still hoping for a happy ending. I totally understand your fear, but I think you can be more optimistic. Some women do a few banking cycles if you can afford it. Once the blastocysts are formed, embryologists remove cells from each blastocyst and send them to be tested, or perform the tests in-house. Both situations can be emotionally difficult to endure. Preimplantation genetic testing is meant to tell you if your embryo has a specific genetic abnormality or not. As the chart below shows, a relatively small percentage of embryos developed during IVF qualify as "chromosomally-normal" or "euploid." PGS test results are available within 7-10 days. At the start I was only getting max 4 eggs retrieved per cycle (1 blast), on my 6th I was up to 18 eggs retrieved and 4 blasts. The effect of maternal age on the possibility of retrieving at least one normal embryo was similar, with a rapid exponential decline (R2 14 0.986). About 3 out of 4 of these euploid embryos will implant on average (75 percent implantation rate), with high pregnancy rates per transfer. As displayed in the figure below, the percentage of genetically abnormal embryos increases significantly after women reach the age of 35. Hello, I am 35 and had 7 blastocyst sent to PGS and 3 came back normal. PGT-A: Preimplantation Genetic Testing for Aneuploidy 10 Important Factors To Consider, IVF Success Rates Over 40 with Own Eggs and Donor Eggs, Male factor infertility IVF success rates. This number indicates the blastocyst development stage or the degree of the expansion of the embryo's cavity. As part of the normal IVF treatment, women take medications to stimulate their ovaries and help the eggs to mature. This article provides an overview of IVF withPGS/PGD success rates. Lap #1 in 2005 (mild endo), Lap #2 in 2013 (confirmed no regrowth), IVF #1 (March 2018) - 4 eggs retrieved, 1 blast - 3BC (not tested), fresh transfer, BFN, Changed clinics - now @ MFC / Dr Virro - referred for immune testing, Dr wants to first get 2-3 PGS normal embryos before doing immune testing, May 2018-May 2019: 6 more IVF cycles.. 12 embryos, 3 PGS normal (5AB, 3AB, 4BC), 1 mosaic (4BB), 1 too small to test (3BC), 7 abnormal, Immune testing revealed low leukocyte antibodies (1%), May-June 2019: LIT #1 (x2 treatments) with DH - minor improvement, July-Sept 2019: LIT #2 with donor (x3 treatments) - again minor improvement, Oct-Nov 2019: LIT #3 with new donor (x2 treatments) - not going to wait for the results, proceeding with transfer in December 2019, Community Forum Software by IP.BoardLicensed to: IVFCA Fertility Network 2013. most biologically active form of folic acid. Patients often hear PGS-normal embryos have a 60 70% success rate. Its worth noting that the reason for testing is to avoid the transfer of embryos with irregular chromosome counts. Check out mycomplete guide to embryo grading and success ratesto learn more about embryo development, grading and success rates. PGT-A can reveal the gender of an embryo by looking at the two sex chromosomes. Our RE said that any changes in supplements will take at least 3 months to have an effect? At the age of 25, 75% of a woman's eggs are chromosomally normal. For more background info, check out my post onPGS Testing. Munn S, Kaplan B, Frattarelli JL, Child T, Nakhuda G, Shamma FN, Silverberg K, Kalista T, Handyside AH, Katz-Jaffe M, Wells D, Gordon T, Stock-Myer S, Willman S; STAR Study Group. think twice before sharing personal details, foster a friendly and supportive environment, remove fake accounts, spam and misinformation, delete posts that violate our community guidelines, reviewed by our medical review board and team of experts. PGS is also known as pre-implantation genetic testing for an aneuploidy (PGT-A). ***tw*** we transferred one, and I'm 14 weeks along. IVF#1 - BFN. Capalbo et al. Therefore, an embryo with 46 chromosomes is called a euploid embryo and is considered normal. But what about the women who didnt get blasts? Also, keep in mind that pregnancy rates will also depend on the overall egg quality of the eggs retrieved during one . Segmental aneuploids: the main source for PGT-A false positives? The pregnancy rate for embryo transfers following PGT is 70 to 75 percent in women under the age of 40. Theper retrievalstatistic helps to see the chancesbefore PGStesting. There isnt much data on it, and 2 of the 3 studies found no difference, so it isnt exactly clear. On my 6th retrieval, we finally got 2 PGS normal and then did one more cycle we got 1 more. FZMM 5 yr. ago The maximum post-PGS live birth rate for women younger than 35 is 60%. Have a prescription for vitamin d and forpropylthiouracil. It also depends on what day embryo you have, day 5 - 80% chance of success, day 6 - 70% chance of success, day 7 - 60% chance of success. In two of these embryos all reanalysed cells were normal diploid, i.e. If you do CCS or CGH testing (the type of PGD that's normally done on blasts, which tests all 23 chromosomes), the error rate (false negative or positive) is only about 3%. Entire Website 2003 - 2020Karande and Associates d/b/a InViaFertility Specialists, Age and Number of Eggs Obtained After Preimplantation Genetic Screening, Intro to Assisted Reproductive Technologies, Demko et al addressed this very issue in a recent publication, make an appointment at one of InVia's four Chicago area fertility clinics, Top 10 Questions Our Fertility Nurses Answer, Don't Delay Frozen Embryo Transfer After Failed IVF. Infertility issues are so heartbreaking already! In a small study,Bradley et al. However, mosaic embryos do have as high of live birth rates compared to euploid embryos. IVF statistics single euploid embryo transfer program 2021 (2017)found a reduction in embryo survival (from 98% to 93%) and a reduction inlive birth rate(50% to 27%). We sent the 3 blasts together for PGS and none of them were normal which was heartbreaking. In all her years of medical training, she had never encountered some of the hard truths of female infertility, including the fact that for women older than 42, nearly 90 percent of the embryos they produce are aneuploid. I know how you feel, but it's awesome you got 2. But that is on a per-transfer basis. 2 5-day blasts transferred (Assisted Hatching -AH used).
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